文章摘要
| 周建中,马勇,殷韶健,孙士杰.中药脊髓康治疗大鼠脊髓损伤的实验研究[J].江西中医药大学学报,2009,21(2):71-74. |
| 中药脊髓康治疗大鼠脊髓损伤的实验研究 |
| Experiment study of Jisuikang on treatment of rat after spinal cord injury |
| 投稿时间：2008-10-16 |
| DOI： |
| 中文关键词: 脊髓损伤；脊髓康；中药；实验研究 |
| 英文关键词: spinal cord injury; Jisuikang; traditional Chinese medicine; treatment |
| 基金项目:基金项目：江苏省中医药局资助项目(HD5072) |
| 周建中1,马勇2,殷韶健1,孙士杰1 |
| 1.南京中医药大学2006级硕士研究生,南京210029；2.南京中医药大学第一临床医学院骨伤科研究所,南京210029 |
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| 中文摘要: |
| 目的：观察脊髓康对脊髓损伤大鼠的治疗作用及其作用是否有剂量依赖性，并与强的松进行对照。方法：SD大鼠72只随机分为6组(n=12)。造模后脊髓康高、中、低剂量组予脊髓康25、12.5、6.25 g生药/kg体重；强的松组予强的松0.06g/kg体重。正常组及模型组予等量蒸馏水。测定血液及损伤脊髓中IL-10、INF-α、NO、NOS、SOD、MDA含量；全血粘度；损伤脊髓病理学检查。结果：血液中TNF-α含量升高，脊髓康各剂量组相对模型组降低。血液中IL-10水平下降，强的松组和脊髓康各剂量组相对模型组有 |
| 英文摘要: |
| Objective: To observe the effect of Jisuikang on treatment of rats after empirical spinal cord injury,and understand whether has dose-dependent effect or not,and perform positive control with prednisone.Methods: Seventy-two SD rats were separated randomly into six groups (n=12).The rats in the Jisuikang high-dose group,middle-dose group and low-dose group were given Jisuikang 25、12.5、6.25 g/kg for 14 days.The rats in the prednisone group were given 0.06 g/kg prednisone for 7 days.The rats in normal and model group were given distilled water of the same volume.After one hour of last administration,collecting the blood in rats’ fossa orbitalis of every group,testing the content of IL-10、TNF-α and NO、SOD、NOS、MDA in the blood; testing whole blood viscosity; testing the content of NO、SOD、NOS、MDA in the injuried spinal cord tissue.Spinal cord histopathologic examination was done ater determination.Results: After modeled,the content of TNF-α in the blood of rats was raised compared with normal group,and this in Jisuikang high-dose group(P＜0.01),middle-dose group(P＜0.05) and low-dose group(P＜0.01) was decreased compared with model group.After modeled the content of IL-10 in the blood of rats was obviously decreased compared with normal group,and this in Jisuikang high-dose group,middle-dose group,low-dose group and prednisone group was significant difference as compared with model group(P＜0.01).After modeled,the rats’ whole blood viscosity was significant difference as compared with normal group.And this in prednisone group had no difference as compared with model group.But this in Jisuikang high-dose group and middle-dose group was lower than this in model group.After modeled,the content of SOD in the blood was obviously decreased but MDA,NO and NOS raised.The content of SOD in Jisuikang high-dose group and middle-dose group was higher than this in model group,but the MDA content in the two groups was lower than this in model group.The content of NOS in Jisuikang high-dose group,middle-dose group and low-dose group was lower than this in model group.The content of NO in Jisuikang high-dose group and middle-dose group was lower than this in model group.Compared with model group,the MDA content in prednisone group was lower,but the SOD content had no change.After modeled,the content of SOD in the injuried spinal cord tissue was obviously decreased but MDA,NO and NOS raised.The content of SOD in Jisuikang high-dose group was higher than model group.The content of MDA and NOS in Jisuikang high-dose group and middle-dose group were both lower than these in model group.The content of NO in Jisuikang high-dose group,middle-dose group and low-dose group was lower than this in model group.Compared with model group,the MDA content in the injuried spinal cord tissue in prednisone group was lower,but the SOD content had no change.Results of pathological examination: The pathological change of spinal cord tissue in Jisuikang every group was lighter,especially in Jisuikang high-dose group.The pathological change in prednisone group was similar as this in Jisuikang high-dose group and middle-dose group.Conclusion: Jisuikang can inhibit NOS expressing and degrade the content of NO.Jisuikang can degrade the content of MDA and raise the content of SOD.Jisuikang can improve the index of hemorheology.Jisuikang can degrade the content of TNF-α.Jisuikang can raise the content of IL-10.Jisuikang can prevent secondum change of spinal cord injury,promote recovery of spinal cord tissue and regeneration of nerve tissue.The therapeutic effect of Jisuikang high-dose group was more significant than Jisuikang middle-dose group. |
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