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文章摘要
.HPLC 双波长法同时测定甘草药材中 5 种活性成分含量 及主成分分析[J].江西中医药大学学报,2024,36(2):88-93.
HPLC 双波长法同时测定甘草药材中 5 种活性成分含量 及主成分分析
Simultaneous Contents Determination of Five Components in Glycyrrhiza by Dual-wavelength HPLC and Principal Component Analysis
  
DOI:10.20140/j.2095-7785.2024.02.22
中文关键词: 甘草;双波长;HPLC ;含量测定;主成分分析
英文关键词: Glycyrrhiza; Dual-wavelength; HPLC; Content Determination; Principal Component Analysis
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中文摘要:
      目的:建立 HPLC 双波长法同时测定甘草中甘草苷、甘草素、异甘草苷、异甘草素和甘草酸含量,为甘草质量的综合 评价提供参考。方法:采用 Zorbax SB-C18 色谱柱(250 mm×4.6 mm,5 μm),以 0.1% 磷酸 - 乙腈为流动相,梯度洗脱,检 测波长为 248 nm 和 362 nm,柱温为 30 ℃,流速为 1.0 mL/min。使用统计学软件 SPSS 21.0 对 15 批甘草的测定结果进行主成 分分析。结果:5 种活性成分分离效果良好,精密度、重复性、稳定性、线性关系、加样回收率和耐用性均良好。主成分分析 可知,新疆甘草总体质量较好并且差异较小。结论:本法可同时测定 5 种成分的含量且可靠简便,结合主成分分析可为甘草 质量的综合评价提供参考。
英文摘要:
      Objective: To establish a dual-wavelength HPLC method for simultaneous determination of liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin and glycyrrhizic acid in glycyrrhiza, providing reference for comprehensive evaluation of the quality of glycyrrhiza. Methods: The HPLC separation was performed on a Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm) with gradient elution of 0.1% phosphoric acid in water-acetonitrile, and peaks were detected at 248 nm and 362 nm, the column temperature was maintained at 30 ℃ the flow rate was 1.0 mL/ min, The determination results of 15 batches glycyrrhiza uralensis were analyzed by SPSS 21.0 with principal component analysis. Results: The five active ingredients were well separated with good precision, reproducibility, stability, linearity, spiked recoveries and durability. The principal component analysis showed that the overall quality of Xinjiang Glycyrrhiza glabra was good and the differences were small. Conclusion: The method can be used to determine the contents of the five components simultaneously, and it is reliable and simple, and combined with the principal component analysis, it can provide a reference for the comprehensive evaluation of the quality of Glycyrrhiza glabra.
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